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Assignment 2
 

Assignment 2

Another factor that strongly influences enzyme activity in living cells is the pH of the environment in which the enzyme is designed to function. For example, in humans, a cytoplasmic protein in a skin cell is surrounded by a different fluid environment at a different pH than a membrane-bound enzyme like invertase with an active site that projects out into the lumen of the small intestine. The exercises in Assignment 2 of the EnzymeLab are designed to help you understand the effect of pH on enzyme activity by studying invertase activity over a range of different pH values from acidic to basic conditions. Please reference and complete Assignment 2 and answer the questions below.
1 .       Formulate a hypothesis to predict the effect of pH on invertase activity. and enter it below.  



2 .       What is the optimal pH for invertase activity? Do the results of this experiment support or refute your hypothesis? Why or why not? Explain your answers below.  



3 .       Why and how do pH changes affect invertase activity?  



4 .       If you were to carry out these pH experiments at a higher or lower temperature, what effect would this have on the pH optimum for invertase? Formulate a hypothesis and then test your hypothesis. What did you discover? Explain your results below.  








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