In a molecular biology laboratory, a student obtained competent E. coli cells and used a common transformation procedure to induce the uptake of plasmid DNA with a gene for resistance to the antibiotic kanamycin. The results above were obtained.

If a student wants to verify that transformation has occurred, which of the following procedures should she use?

a. Spread cells from Plate I onto a plate with LB agar; incubate.
b. Spread cells from Plate II onto a plate with LB agar; incubate.
c. Repeat the initial spread of –kanR cells onto plate IV to eliminate possible experimental error.
d. Spread cells from Plate II onto a plate with LB agar with kanamycin; incubate.
e. Spread cells from Plate III onto a plate with LB agar and also onto a plate with LB agar with kanamycin; incubate.

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